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Presentation type	E-poster
Title	Refining the Diagnostic Technique to Determine Prevalence of Demodex Mites in Eyelash Hair Follicles Within Standard Ophthalmic Practice: A Single-Center Outpatient Clinical Study

Purpose	A high density of Demodex infestation in human eyelids and eyelash follicles has been implicated in a variety of ocular surface conditions. However, Demodex infestation often goes undiagnosed and untreated, due to an overlap in signs and symptoms with other sources of ocular surface inflammation. We sought to refine the diagnostic technique for Demodex infestation and determine prevalence of Demodex mites in the hair follicles of eyelashes from patients in a standard ophthalmic practice.
Methods	Patients recruited from a single outpatient ophthalmology clinic were examined for the presence of Demodex mites. During anterior segment biomicroscopic examination, investigators searched for cylindrical dandruff and collarettes. Presence of Demodex, cylindrical dandruff and collarettes, time taken to identify the first Demodex mite, mean number of Demodex mites per hair shaft, and patient-reported symptoms of blepharitis were recorded and analyzed to determine potential correlations.
Results	A total of 173 patients were recruited, of whom 106 were included in Demodex prevalence analyses. Demodex infestation was identified in 94% patients, with a mean time of 2 minutes and 20 seconds taken to find the first Demodex mite and a mean number of 3 mites identified per hair shaft. Presence of Demodex significantly correlated with the presence of cylindrical dandruff (98/106 patients), but not with the presence of collarettes (62/106 patients) or symptoms of blepharitis (15/106 patients).
Conclusion	These results highlight the pervasiveness of Demodex infestation, predicted by the presence of cylindrical dandruff. They support the use of a non-invasive diagnostic technique to allow fast, simple identification of Demodex in outpatient clinical practice.
Conflict of interest	No

Authors 1

Last name	BOEL
Initials	C
Department	UZ Brussel
City	Brussel

Authors 2

Last name	Goezinne
Initials	F
Department	Eyescan Rijswijk
City	Rijswijk