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This abstract is assigned to session AOB Poster session 1 - Glaucoma – Posterior segment/Retinal disease

Title	Intravitreal injected fluid dispersion: importance of injection technique.
Abstract Nr.	1025

Purpose	To evaluate the dispersion of intravitreal injected solutions and to investigate the influence of varying injection techniques.
Methods	Prospective experimental study using enucleated porcine eyes and ultra-high resolution computed tomography (UHRCT) scanning to visualize iomeprol intravitreal dispersion. 60 porcine eyes were divided over 12 different groups according to the injection procedure: fast (2 sec) or slow (10 sec) injection speed and needle tip location (6 and 12 mm needle shaft insertion or premacular needle tip placement verified by indirect ophthalmoscopy). For each of these combinations eyes were either injected the combination of V20I (which is an analogue of ocriplasmin) and iomeprol or iomeprol alone. Distance to the macula and volume measurements were performed at 1, 2, 3 and 5 hours after injection.
Results	Sixty fresh enucleated porcine eyes were injected with iomeprol or a combination of iomeprol and V20I. The measured contrast bolus volume increases slowly over time to an average of 0.70 (p=0.03), 1.04 (P=0.006) and 0.79 (p=0.0001) cm³ 5 hours after the injection for 6, 12mm and premacular intravitreal injections, respectively. The distance to the macular marker was significantly lower for premacular injections in respect to 6 mm needle shaft insertion depths.
Conclusion	UHRCT with 3-D reconstruction offers the possibility to study the dispersion of intravitreal injected solutions in a non-invasive manner. Premacular intravitreal injections under indirect funduscopic guidance assured a targeted contrast delivery.
Conflict of interest	Yes
Details of conflicting interests	Peter Stalmans receives lecture fees from Alcon, DORC, Haag-Streit and Thrombogenics; is a consultant for Alcon, Bausch&Lomb, DORC, Nano-Retina, Zeiss and Vitreq; and receives grant support from Thrombogenics.

Authors 1

Last name	WILLEKENS
Initials	K
Department	UZ Leuven
City	Leuven

Authors 2

Last name	Reyns
Initials	G
Department	ThromboGenics NV
City	Leuven

Authors 3

Last name	Diricx
Initials	M
Department	ThromboGenics NV
City	Leuven

Authors 4

Last name	Vanhove
Initials	M
Department	ThromboGenics NV
City	Leuven

Authors 5

Last name	Noppen
Initials	B
Department	ThromboGenics NV
City	Leuven

Authors 6

Last name	Coudyzer
Initials	W
Department	UZ Leuven
City	Leuven

Authors 7

Last name	Ni
Initials	Y
Department	UZ Leuven
City	Leuven

Authors 8

Last name	Feyen
Initials	JHM
Department	ThromboGenics NV
City	Leuven

Authors 9

Last name	Stalmans
Initials	P
Department	UZ Leuven
City	Leuven