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Type de présentation	E-poster
Titre	Next-Generation Sequencing for Excised Conjunctival Naevi: Does More Data Equal Better Care?

But	To evaluate the benefits and risks of next-generation sequencing (NGS) in excised conjunctival naevi in children.
Méthodes	Two pediatric cases of excised conjunctival naevi were reviewed.
Résultats	In the first case (boy - 10y), NGS identified a BRAF V600E pathogenic variant in a histologically benign conjunctival naevus. Although BRAF V600E is also found in benign conjunctival naevi, the presence of an involved surgical margin caused uncertainty and concern for the patient’s parents. Following a multidisciplinary consultation with the department of Pathology and Medical Genetics, it was unanimously decided to classify this lesion as benign. Consequently, no wider resection or adjuvant therapy was performed. In the second case (girl – 13y), a MAP2K1 mutation and a pathogenic BRCA1 variant were detected in a melanocytic nevus. MAP2K1 alterations may occur in both benign and malignant melanocytic lesions. Further analysis showed a low BRAC1 variant allele frequency, which classified the mutation as somatic and not part of a germline mutation.
Conclusion	NGS can provide valuable information about conjunctival melanocytic lesions. However, more molecular data does not automatically result in better clinical care. In histologically confirmed benign conjunctival naevi, the presence of BRAF V600E or MAP2K1 mutations does not always allow one to distinguish between benign and malignant disease. Therefore, NGS may increase uncertainty for both patients and surgeons. A multidisciplinary approach is essential for proper patient counseling.
Conflit d'intérêt	Non

Auteurs 1

Nom	VANDERMEULEN
Initiales	M
Institut	Department of Ophthalmology, Ghent University Hospital
Ville	Ghent

Auteurs 2

Nom	DIMITRI
Initiales	R
Institut	Department of Ophthalmology, Ghent University Hospital
Ville	Ghent