Titre | Long-term results of standardized, non-xenogenic, cultivated limbal epithelial stem cell transplantations. |
Abstract Nr. | 3004 |
But | To evaluate the long-term success rate of transplanted non-xenogenic, bio-engineered, composite grafts of cultured limbal epithelial cells on standardized amniotic membranes in patients with limbal stem cell deficiency. |
Méthodes | We report the extended outcome of standardized cultivated limbal stem cell transplantations of a phase I/II clinical trial. Between July 2008 and May 2012, 21 patients with partial (n=3) or total (n=18) limbal stem cell deficiency received either autologous (n=15) or allogenic (n=6) stem cell grafts, using a protocol free from xenogenic products and a reduced manipulation surgical technique. The outcome measures were compared with short-term results (mean follow-up of 22 months) of the same trial, in which a 67% success rate was seen. |
Résultats | Five patients were lost to follow-up and 2 patients were excluded because of unrelated side effects. The remaining 14 patients had a mean follow-up of 68 months (range 29-100 months). Of these, 7 (50%) patients were graded as anatomical success. Three of the 7 patients retained a persistent intact epithelium without corneal neovascularization and were subclassified as ‘total’ anatomical success. Four of the 7 patients were subclassified as ‘partial’ anatomical success meaning a complete epithelialization with a mild revascularization, but less than ad admission. There was no significant improvement of visual acuity, pain or photophobia in the total cohort. |
Conclusion | Cultivated limbal epithelial stem cell transplantation offers a safe alternative in the reconstruction of the anterior cornea. However, compared to early post-operative results, there is a decline of anatomical and functional success over time. |
Conflit d'intérêt | Non |
Nom | BEHAEGEL |
Initiales | J |
Institut | UZA |
Ville | Edegem |
Nom | Ní Dhubhghaill |
Initiales | S |
Institut | UZA |
Ville | Edegem |
Nom | Koppen |
Initiales | C |
Institut | UZA |
Ville | Edegem |
Nom | Leysen |
Initiales | I |
Institut | UZA |
Ville | Edegem |
Nom | Tassignon |
Initiales | MJ |
Institut | UZA |
Ville | Edegem |
Nom | Zakaria |
Initiales | N |
Institut | UZA |
Ville | Edegem |