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Titre	Validation of an antiretinal antibody detection strategy for the diagnosis of autoimmune retinopathies.
Abstract Nr.	1050

But	To assess the reliability of detection of antiretinal antibodies (ABs) in serum to diagnose autoimmune retinopathies (AIR) of neoplastic and non-neoplastic origin.
Méthodes	Study samples: sera from suspected AIR patients (n=17), healthy controls (n=10) and patients with antinuclear ABs (ANA) (n=5). Indirect Immunofluorescence (IIF) on primate retina (Euroimmun®) and Western Blotting (WB) against human retinal proteins (homemade) were performed prospectively and compared to patterns obtained with commercial ABs against recoverin, α-enolase, IRBP and TRMP-1. Anti-recoverin ABs were also identified by immunodot (Euroimmun®).
Résultats	At 1/10 dilution, several fluorescent structures were seen with normal sera, but at 1/100 all IIF were negative in healthy controls. Patients with ANA showed a positive signal in the nuclear layers. Among screened AIR samples (1/100), 7 were negative, 4 displayed a positive signal at the level of photoreceptors and 2 showed a positive signal at the level of bipolar cells. 1 of these samples, from a patient with a confirmed melanoma-associated retinopathy, showed a band at 24 kDa by WB. 4 samples showed a speckled pattern in the inner and outer nuclear layers. 2 of these were probably due to a non-specific cross-reaction with ANA.
Conclusion	Screening for antiretinal ABs revealed different patterns on primate retina. At this stage of the study, their clinical significance remains uncertain. WB can help identify the target antigens.

Auteur 1

Nom	DRAGANOVA
Initiales	D
Institut	Dpt. of Ophtalmology, CHU Saint Pierre, CHU Brugmann
Ville	BXL

Auteur 2

Nom	Debaugnies
Initiales	F
Institut	Laboratory of Immunology, CHU Brugmann
Ville	BXL

Auteur 3

Nom	Postelmans
Initiales	L
Institut	Dpt. of Ophtalmology, CHU Brugmann
Ville	BXL

Auteur 4

Nom	Caspers
Initiales	L
Institut	Dpt. of Ophtalmology, CHU Saint Pierre
Ville	BXL

Auteur 5

Nom	Willermain
Initiales	F
Institut	Dpt. of Ophtalmology, CHU Saint Pierre, CHU Brugmann
Ville	BXL

Auteur 6

Nom	Corazza
Initiales	F
Institut	Laboratory of Immunology, CHU Brugmann
Ville	BXL