Titre | Therapeutic potential of placental growth factor (PlGF) inhibition in scar formation after glaucoma filtration surgery |
But | We checked the hypothesis that placental growth factor (PlGF) may be a target for improvement of the outcome of glaucoma filtration surgery (GFS). |
Méthodes | The effect of the anti-murine PlGF-antibody (5D11D4) was investigated in a mouse model of GFS. Immediately after surgery 5D11D4 (5.2µg) or 1C8, an irrelevant mouse IgG antibody (4.8 µl), was injected in the anterior chamber (n=10 eyes for both groups). An anti-murine VEGF-R2 antibody (DC101) was used as a positive control (6.2 µg; n=10). Mice were killed on post-operative day 8. Treatment outcome was studied by clinical investigation of the bleb and by immunohistological stainings. All antibodies were kindly provided by ThromboGenics NV. |
Résultats | Treatment using the anti-PlGF antibody significantly improved surgical outcome by increasing bleb survival and bleb area with 29% compared to negative control (1C8). Postoperative inflammation and angiogenesis was reduced during the first days after surgery and collagen deposition was decreased at later stages. A single administration of anti-VEGF-R2 also significantly improved bleb area with 7% as compared to 1C8, but had no effect on bleb survival. Inhibition of VEGF-R2 only reduced angiogenesis and collagen deposition, but did not influence inflammation. |
Conclusion | Targeting PlGF with an inhibitory monoclonal antibody is efficacious in improving GFS outcome, possibly even more effectively than inhibition of VEGF-R2, due to its additional effect on inflammation. These results render PlGF an appealing target for ocular wound healing and point to the potential therapeutic benefits of PlGF-inhibition. |
Nom | VAN BERGEN |
Initiales | T |
Institut | KUL |
Ville | Leuven |
Nom | Jonckx |
Initiales | B |
Institut | ThromboGenics |
Ville | Heverlee |
Nom | Hollanders |
Initiales | K |
Institut | KUL |
Ville | Leuven |
Nom | Sijnave |
Initiales | D |
Institut | KUL |
Ville | Leuven |
Nom | Van de Velde |
Initiales | S |
Institut | KUL |
Ville | Leuven |
Nom | Vandewalle |
Initiales | E |
Institut | KUL |
Ville | Leuven |
Nom | Moons |
Initiales | L |
Institut | KUL |
Ville | Leuven |
Nom | Stassen |
Initiales | JM |
Institut | ThromboGenics |
Ville | Heverlee |
Nom | Stalmans |
Initiales | I |
Institut | KUL |
Ville | Leuven |