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TitelDecoding growth factor profiles of platelet-derived eye drops for ocular surface disease: a systematic review of preparation, preservation and comparison with serum eye drops.
DoelTo systematically review and analyze the literature on growth factor (GF) composition in autologous and allogeneic platelet-derived eye drops (PDED), and compare them with serum eye drops (SED). Additionally, to evaluate the impact of preparation and storage methods on GF levels.
MethodesA systematic literature review was conducted in MEDLINE, Embase, Web of Science, and Cochrane Library according to the PRISMA guidelines. Studies reporting the GF levels (EGF, FGF, HGF, IGF-1, NGF, PDGF(-AA, -AB, -BB), TGF-β1, VEGF(-A)) of any type of PDED (e.g. PRGF, PRP, platelet lysate) with or without comparison to SED, were included. Absolute GF levels and relative comparison to SEDs, along with data on preparation (activation, centrifugation, filtration, dilution) and storage were extracted.
ResultatenOur review identifies substantial variability in reported GF levels, preparation protocols, and terminology. GF levels were unaffected by differences in centrifugation and filtration, but were influenced by activation method and heat treatment. Comparison with SED exhibits distinct GF profiles, with some factors (EGF, FGF, NGF, PDGF, TGF-β1) being higher in PDED and others (VEGF, HGF) in SED. Both short term storage at 4°C and longer frozen storage maintain stable GF content, with lyophilization representing a promising alternative.
ConclusiePDED represent a valuable biological treatment for ocular surface disorders. However, marked heterogeneity in preparation, GF levels and terminology impedes uniform translation into clinical practice. With this review, we aim to highlight patterns in PDED production and its impact on GF levels, offering direction for further research focused on optimizing preparation protocols.
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Auteurs: Cools L (1,2,3), Orbie S (4), Van Remoortel H (5,6), Six KR (3), Feys HB (3,7), Saelens
IEY (1,2), Delbeke H (1,2)
1. Department of Ophthalmology, University Hospitals Leuven, Leuven, Belgium.
2. Department of Neurosciences, Research Group Ophthalmology, Biomedical Sciences
Group, KU Leuven, Leuven, Belgium.
3. Transfusion Research Center, Belgian Red Cross Flanders, Ghent, Belgium.
4. Faculty of Medicine, KU Leuven, Leuven, Belgium
5. Center for Evidence-Based Practice, Belgian Red Cross Flanders, Mechelen, Belgium.
6. Department of Public Health and Primary Care, Leuven Institute for Healthcare Policy, KU
Leuven, Leuven, Belgium.
7. Department of Diagnostic Sciences, Faculty of Medicine, Ghent University, Ghent,
Belgium.
BelangenverstrengelingNee
Auteurs 1
NaamCOOLS
InitialenL
Auteurs 2
NaamOrbie
InitialenS
Auteurs 3
NaamVan Remoortel
InitialenH
Auteurs 4
NaamSix
InitialenKR
Auteurs 5
NaamFeys
InitialenHB
Auteurs 6
NaamSaelens
InitialenIEY
Auteurs 7
NaamDelbeke
InitialenH
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