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TitelValidation of an antiretinal antibody detection strategy for the diagnosis of autoimmune retinopathies.
Abstract Nr.1050
DoelTo assess the reliability of detection of antiretinal antibodies (ABs) in serum to diagnose autoimmune retinopathies (AIR) of neoplastic and non-neoplastic origin.
MethodesStudy samples: sera from suspected AIR patients (n=17), healthy controls (n=10) and patients with antinuclear ABs (ANA) (n=5). Indirect Immunofluorescence (IIF) on primate retina (Euroimmun®) and Western Blotting (WB) against human retinal proteins (homemade) were performed prospectively and compared to patterns obtained with commercial ABs against recoverin, α-enolase, IRBP and TRMP-1. Anti-recoverin ABs were also identified by immunodot (Euroimmun®).
ResultatenAt 1/10 dilution, several fluorescent structures were seen with normal sera, but at 1/100 all IIF were negative in healthy controls. Patients with ANA showed a positive signal in the nuclear layers. Among screened AIR samples (1/100), 7 were negative, 4 displayed a positive signal at the level of photoreceptors and 2 showed a positive signal at the level of bipolar cells. 1 of these samples, from a patient with a confirmed melanoma-associated retinopathy, showed a band at 24 kDa by WB. 4 samples showed a speckled pattern in the inner and outer nuclear layers. 2 of these were probably due to a non-specific cross-reaction with ANA.
ConclusieScreening for antiretinal ABs revealed different patterns on primate retina. At this stage of the study, their clinical significance remains uncertain. WB can help identify the target antigens.
Auteur 1
NaamDRAGANOVA
InitialenD
InstituutDpt. of Ophtalmology, CHU Saint Pierre, CHU Brugmann
StadBXL
Auteur 2
NaamDebaugnies
InitialenF
InstituutLaboratory of Immunology, CHU Brugmann
StadBXL
Auteur 3
NaamPostelmans
InitialenL
InstituutDpt. of Ophtalmology, CHU Brugmann
StadBXL
Auteur 4
NaamCaspers
InitialenL
InstituutDpt. of Ophtalmology, CHU Saint Pierre
StadBXL
Auteur 5
NaamWillermain
InitialenF
InstituutDpt. of Ophtalmology, CHU Saint Pierre, CHU Brugmann
StadBXL
Auteur 6
NaamCorazza
InitialenF
InstituutLaboratory of Immunology, CHU Brugmann
StadBXL
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