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Deze abstract is toegekend aan sessie FRO: Fund for Research in Ophthalmology
TitelFRO: Subconjunctival bevacizumab enhances the antifibrotic effect of MMC and allows to reduce its exposure time to improve safety
Abstract Nr.1030
DoelTo determine the most optimal administration route of bevacizumab after GFS and to investigate whether reducing the exposure time and/or dose of MMC in combination with bevacizumab could improve surgical outcome with a lower incidence of side effects.
MethodesIn the first experiment, mice were operated and received a subconjunctival (SC), intracameral (IC) or intravitreal (IV) injection of bevacizumab (25µg). Bevacizumab plasma levels were measured using ELISA. In the second experiment, the combination of MMC and bevacizumab was compared to MMC in operated mice. Surgical sponges soaked in MMC 0.02% and 0.01% were investigated and exposed to the sclera for 1 or 2 min. Treatment outcome was studied by clinical investigation of the bleb.
ResultatenTreatment using a SC, IC or IV bevacizumab equally improved surgical outcome. Importantly, bevacizumab was detected at relatively high levels in plasma shortly after IV injection, whereas minimal bevacizumab absorption was detected only from day 4 after SC or IC administration. Administration of SC bevacizumab combined with 1 or 2 min of MMC 0.02% equally improved bleb area, as compared to MMC 0.02% alone. The combination of bevacizumab and 1 min exposure of MMC 0.01% also significantly improved surgical outcome (versus 1 min MMC 0.01%), although to a lesser extent than the combination with MMC 0.02% for 1 min. Importantly, 25% of the eyes treated for 2 min with MMC showed corneal toxicity, whereas this was not the case after 1 min of administration.
ConclusieAdjunctive subconjunctival bevacizumab allows to reduce the administration time of MMC 0.02%, thereby eliminating its toxic effects on the cornea while maintaining the beneficial effects on surgical outcome.
Auteur 1
NaamVAN BERGEN
InitialenT
InstituutKUL
StadLeuven
Auteur 2
NaamVANDEWALLE
InitialenE
InstituutKUL
StadLeuven
Auteur 3
NaamMOONS
InitialenL
InstituutKUL
StadLeuven
Auteur 4
NaamSTALMANS
InitialenI
InstituutKUL
StadLeuven
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