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Presentation type	Oral presentation
Title	Decoding growth factor profiles of platelet-derived eye drops for ocular surface disease: a systematic review of preparation, preservation and comparison with serum eye drops.

Purpose	To systematically review and analyze the literature on growth factor (GF) composition in autologous and allogeneic platelet-derived eye drops (PDED), and compare them with serum eye drops (SED). Additionally, to evaluate the impact of preparation and storage methods on GF levels.
Methods	A systematic literature review was conducted in MEDLINE, Embase, Web of Science, and Cochrane Library according to the PRISMA guidelines. Studies reporting the GF levels (EGF, FGF, HGF, IGF-1, NGF, PDGF(-AA, -AB, -BB), TGF-β1, VEGF(-A)) of any type of PDED (e.g. PRGF, PRP, platelet lysate) with or without comparison to SED, were included. Absolute GF levels and relative comparison to SEDs, along with data on preparation (activation, centrifugation, filtration, dilution) and storage were extracted.
Results	Our review identifies substantial variability in reported GF levels, preparation protocols, and terminology. GF levels were unaffected by differences in centrifugation and filtration, but were influenced by activation method and heat treatment. Comparison with SED exhibits distinct GF profiles, with some factors (EGF, FGF, NGF, PDGF, TGF-β1) being higher in PDED and others (VEGF, HGF) in SED. Both short term storage at 4°C and longer frozen storage maintain stable GF content, with lyophilization representing a promising alternative.
Conclusion	PDED represent a valuable biological treatment for ocular surface disorders. However, marked heterogeneity in preparation, GF levels and terminology impedes uniform translation into clinical practice. With this review, we aim to highlight patterns in PDED production and its impact on GF levels, offering direction for further research focused on optimizing preparation protocols. ------- Auteurs: Cools L (1,2,3), Orbie S (4), Van Remoortel H (5,6), Six KR (3), Feys HB (3,7), Saelens IEY (1,2), Delbeke H (1,2) 1. Department of Ophthalmology, University Hospitals Leuven, Leuven, Belgium. 2. Department of Neurosciences, Research Group Ophthalmology, Biomedical Sciences Group, KU Leuven, Leuven, Belgium. 3. Transfusion Research Center, Belgian Red Cross Flanders, Ghent, Belgium. 4. Faculty of Medicine, KU Leuven, Leuven, Belgium 5. Center for Evidence-Based Practice, Belgian Red Cross Flanders, Mechelen, Belgium. 6. Department of Public Health and Primary Care, Leuven Institute for Healthcare Policy, KU Leuven, Leuven, Belgium. 7. Department of Diagnostic Sciences, Faculty of Medicine, Ghent University, Ghent, Belgium.
Conflict of interest	No

Authors 1

Last name	COOLS
Initials	L

Authors 2

Last name	Orbie
Initials	S

Authors 3

Last name	Van Remoortel
Initials	H

Authors 4

Last name	Six
Initials	KR

Authors 5

Last name	Feys
Initials	HB

Authors 6

Last name	Saelens
Initials	IEY

Authors 7

Last name	Delbeke
Initials	H