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This abstract is assigned to session FRO: Fund for Research in Ophthalmology

Title	FRO: Rho Kinase Inhibitor AMA0526 Improves Surgical Outcome in a Rabbit Model of Glaucoma Filtration Surgery
Abstract Nr.	1032

Purpose	To elucidate the effect of a locally acting, selective ROCK inhibitor on the wound healing process and surgical outcome of glaucoma filtration surgery.
Methods	The in vivo effect of topical AMA0526 0.3% TID was investigated in a rabbit model of glaucoma filtration surgery. Treatment outcome was studied by clinical investigation of the bleb area as well as immunohistological analyses for inflammation, angiogenesis and collagen deposition at day 8, 14 and 30 after surgery. Vehicle was used as control in seperate groups.
Results	AMA0526 significantly improved bleb area and survival compared to vehicle treated eyes. Immunohistological analyses showed significant reduction of inflammation, angiogenesis and collagen deposition after treatment with the ROCK inhibitor. Compared to vehicle, inflammation was decreased by 33% at 8 days, angiogenesis was reduced by 52% on day 8 and by 29% at 14 days, and collagen deposition by 30% on surgical day 30.
Conclusion	This study shows that AMA0526 is able to improve glaucoma surgery outcome in rabbits. In addition to improved bleb area, AMA0526 led to decreased inflammation, angiogenesis and fibrosis. This study can open new perspectives for a safer and more efficient glaucoma surgery.

Authors 1

Last name	VAN DE VELDE
Initials	S
Department	KUL
City	Leuven

Authors 2

Last name	Van Bergen
Initials	T
Department	KUL
City	Leuven

Authors 3

Last name	Vandewalle
Initials	E
Department	KUL/UZ
City	Leuven

Authors 4

Last name	Castermans
Initials	K
Department	Amakem
City	Diepenbeek

Authors 5

Last name	Kindt
Initials	N
Department	Amakem
City	Diepenbeek

Authors 6

Last name	Moons
Initials	L
Department	KUL
City	Leuven

Authors 7

Last name	Stalmans
Initials	I
Department	KUL/UZ
City	Leuven